Fundamentals of Energy-dispersive X-ray spectroscopy (EDS)

Fundamentals of Energy-dispersive X-ray spectroscopy (EDS)

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X-ray is a kind of electromagnetic wave, the same as light. The wavelength of visible light is 400 to 800nm, while the wavelength of x-ray is much shorter (higher energy), at 0.001nm to 10nm, and is known to have strong penetrating power.

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What are the main differences between an SEM, an ESEM, an SEM-FIB and an (S)TEM?

What are the main differences between an SEM, an ESEM, an SEM-FIB and an (S)TEM?

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The Scanning Electron Microscope (SEM) produces images by probing the specimen with a focused electron beam that is scanned across a rectangular area of the specimen (raster scanning).

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Interpretation steps of a NMR spectrum

Interpretation steps of a NMR spectrum

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Nuclear Magnetic Resonance (NMR) spectroscopy is an incredibly powerful tool for characterizing molecular structures. When submitting to the FDA or other regulatory agencies, full structural characterization by NMR provides crucial evidence of compound identity. A combination of 1-dimensional and 2-dimensional NMR experiments are necessary for complete confidence in chemical structure.

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Fundamentals of Nuclear Magnetic Resonance Spectroscopy

Fundamentals of Nuclear Magnetic Resonance Spectroscopy

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Over the past fifty years nuclear magnetic resonance spectroscopy, commonly referred to as nmr, has become the preeminent technique for determining the structure of organic compounds. Of all the spectroscopic methods, it is the only one for which a complete analysis and interpretation of the entire spectrum is normally expected. Although larger amounts of sample are needed than for mass spectroscopy, nmr is non-destructive, and with modern instruments good data may be obtained from samples weighing less than a milligram. To be successful in using nmr as an analytical tool, it is necessary to understand the physical principles on which the methods are based.

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A to Z of IR Spectroscopy

A to Z of IR Spectroscopy

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Infrared (IR) spectroscopy is based on molecular vibrations caused by the oscillation of molecular dipoles. Bonds have characteristic vibrations depending on the atoms in the bond, the number of bonds and the orientation of those bonds with respect to the rest of the molecule. Thus, different molecules have specific spectra that can be collected for use in distinguishing products or identifying an unknown substance (to an extent.)

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How to analyze TGA thermograms

How to analyze TGA thermograms

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In thermogravimetric analysis (TGA), a sample is continually weighted while heating, as an inert gas atmosphere is passed over it. Many solids undergo reactions that evolve gaseous byproducts. In TGA, these gaseous byproducts are removed and changes in the remaining mass of the sample are recorded. Three variations are commonly employed:

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A to Z of Atomic force microscopy (AFM)

A to Z of Atomic force microscopy (AFM)

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Atomic force microscopy (AFM) is a technique with multiple applications in biology. This method is a member of the broad family of scanning probe microscopy and was initially developed in 1986 by Binnig et al to overcome the disadvantages of the scanning tunneling microscopy (STM) [1]. In the case of STM, only conductive materials can be studied as the resolution is obtained by using a tunneling current between a sharp probe and the sample surface[1]. In contrast, AFM uses small forces on the surface by a probe, thus do not damage samples and can provide information of surface topography of biological materials. AFM soon attracted the attention of the biophysical scientists in biomembrane as well as synthetic membrane research due to its capability of observing biological molecular system with resolution on nanometer scale and its possibility of three dimensional imaging [2].

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Vibrating Sample Magnetometry (VSM), A review

Vibrating Sample Magnetometry (VSM), A review

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Vibrating Sample Magnetometry (VSM) is a measurement technique which allows to
determine the magnetic moment of a sample with very high precision. The aim of this
lab course M106 is to enlarge upon the use of this widespread technique introduced in
the lab course B512, where different ferromagnetic samples were characterized
concerning magnetic hysteresis and demagnetization. Here, we will gain a deeper
understanding of the behavior of magnetic materials and its measurement.

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Interpretation of Vibrating Sample Magnetometer (VSM) analysis

Interpretation of Vibrating Sample Magnetometer (VSM) analysis

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Operation

A sample is made to oscillate using a vibrational unit extended on a rod. The sample is placed between two electromagnetic pieces which are used as the applied field for this this experiment. With the sample oscillating induces a voltage between the search coils which creates a signal to determine the magnetic properties of the sample. Reference coils are used to create a reference signal such that noise generated from the signal can be filtered using a lock-in amplifier [1]. Because the signal and the reference signal are directly related through its voltage and amplitude means that precise measurements can be recorded using a voltmeter. Calibration methods are important to determine the relation between the voltages induced by the magnetic field and the sample and their magnetic properties.

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A to Z of BET analysis

A to Z of BET analysis

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Introduction to BET (Brunauer, Emmett and Teller)

By BET (Brunauer, Emmett and Teller) the specific surface area of a sample is measured – including the pore size distribution. This information is used to predict the dissolution rate, as this rate is proportional to the specific surface area. Thus, the surface area can be used to predict bioavailability. Further it is useful in evaluation of product performance and manufacturing consistency.


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